The tumor microenvironment plays a vital role in cancer development. Multiplex immunostainings allow studying the interaction of different cell types in the tumor microenvironment using a single tissue slide. Though several techniques are available to perform high-plex stainings, they require intensive manual handling, are highly time consuming or not compatible with tissue sections on standard microscope slides.
With the microscope integrated microfluidic system, it is possible to perform fast multiplex stainings including image acquisition without the need to handle the tissue slide. Moreover, due to the sequential nature of the system it would be easily possible to further increase the number of markers in the multiplex staining. Standard off the shelf antibodies can be used for the whole workflow.