Immunofluorescence (IF) is a technique which is widely used in cancer research laboratories which uses antibodies to detect antigens present at the surface of fixed tissue sections with fluorescent-labeled antibodies. The resulting staining can be visualized under a fluorescence microscope.
Visualizing different markers on the same tissue section, thanks to the different microscope filters, is a technique known as Mutiplexed IF, a key research application.
Lunaphore’s microfluidic technology FFeX is able to dramatically reduce IF turnaround times for the first marker to an average of 25-30 minutes for FFPE IF, which greatly impacts the overall multiplexing turnaround times. For instance, a 6-plex plus counterstaining can be performed in approximately 4h30, while a 3-plex plus counterstaining can be done in under 2h30.
An open, Research Use Only device, LabSatTM Research, is currently available, and is able to dramatically reduce Multiplexed IF turnaround times, among other applications, while offering high staining quality and ensuring reproducibility.
Lunaphore is currently developing a high-multiplexing instrument for research laboratories*.