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ISS

In Situ Sequencing

In Situ Sequencing (ISS) is a technique used to identify the presence and spatial location of RNA sequences using Next-Generation Sequencing (NGS), while preserving its original tissue or culture.

ISS has proven useful for the molecular profiling of single cells, similarly to other RNA sequencing techniques, while retaining information about its spatial organization within the tissues. This is important in order to understand the functionality and the processes taking place in these cells. However, differently from regular sequencing techniques, the field of spatially-resolved transcriptomics lacks automation, mainly due to its protocols’ complexity.

Lunaphore’s automated instrument based on a microfluidic technology, FFeX, was tested in a proof-of-concept study, where it performed ISS assays not only showing a dramatic decrease in protocol times, but also a higher detection efficiency. The study identified as well a lower consumption of oligonucleotide reagents with the microfluidic platform, which has a positive impact on its scale-up potential, since the high cost of these reagents is often a barrier for many laboratories.

Lunaphore is currently developing a stand-alone research solution to provide full-automation for multiplexed ISS*.

* Prototype still in development and not for sale.

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