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ISH

In Situ Hybridization

In Situ Hybridization (ISH) is a technique used to identify the presence and location of nucleic acids, such as DNA, mRNA and microRNA sequences, on a fixed tissue sample. Its principle lies on a labeled probe which hybridizes with highly complementary bases present on the target sequence which is being identified.

The detection of the probe can be achieved with both chromogenic and fluorescent techniques, known as CISH (Chromogenic In Situ Hybridization), and FISH (Fluorescence In Situ Hybridization), respectively.

Lunaphore’s microfluidic technology FFeX was tested in a proof-of concept study, showing it was able to carry out FISH protocols decreasing the consumption of the expensive probe solution by a factor 5, with respect to the standard technique, as well as reducing the hybridization time to 4 hours, four times faster than the standard protocol.

Lunaphore is currently developing a stand-alone instrument to provide full-automation for ISH/FISH protocols*.

* Prototype still in development and not for sale.

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