Immunohistochemistry (IHC) is the most widely used cancer biomarker test in tissues. The technique consists in using antibodies to detect antigens thanks to an enzyme-antibody conjugate that reacts with a soluble substrate. The produced insoluble colored product deposits on the surface and can be visualized under a microscope.
The number of biomarkers being identified and stained constantly increases. The need for IHC is therefore growing as hospitals routinely use this method for diagnosing many different types of cancer. One of the advantages of this technique is that the stained samples can be kept and archived without losing intensity in the resulting color. This simplifies the tracking of samples for medical purposes.
Hospital – Pathology
Sample types: Frozen sections or FFPE
Staining time goal*: 10 to 20 minutes