Immunofluorescence (IF) is a technique widely used in research laboratories, which consists in using fluorescently tagged antibodies to detect antigens on tissue samples. The staining can be visualized under a fluorescent microscope.
Fluorescence staining provides numerous research opportunities. Thanks to the different microscope filters used, it’s possible to differentiate several markers on the same tissue section. In addition, the signal intensity can be quantified in order to assess the amount of antibodies present on the sample surface. However, the fluorescence intensity fades over time, therefore making it hard for samples to be re-examined unless they are scanned and stored as an image, limiting its use so far in medical settings.
Research laboratories – University, Pharma/biotech
Sample types: Frozen sections or FFPE
Staining time goal*: 5 – 15min