Hematoxylin & Eosin (H&E) is a non-specific staining technique that utilizes two dyes to produce different colors on cell components, highlighting cell morphology features as well as nuclei in tissues.
This technique is the gold standard as a first line test in diagnostic settings since it is able to provide general information about the tissue easily and in a very short time. However, this technique is often insufficient to distinguish between cells that show similar morphological characteristics and therefore other types of staining are needed. For instance, when biomarker-specific information is needed, techniques such as IHC, IF are required.
Diagnostics in hospitals
Sample types: Frozen or FFPE
Staining time goal*: 3min per marker