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Frozen Section Immunohistochemistry

Immunohistochemistry (IHC) is the most widely used cancer biomarker test for tissues. This technique uses antibodies to detect antigens present at the surface of fixed tissue sections. Thanks to an enzyme-antibody conjugate that reacts with a soluble substrate, the colored product deposits on the tissue surface and can be visualized under a bright-field microscope.

The most common type of fixation for IHC samples is FFPE. Another common technique is the cryosection procedure, which involves freezing the tissue and cutting out frozen sections (FS) with a cryostat. The latter is a faster fixation method and is often used for H&E intraoperative staining to observe tissue morphology, but in order to obtain specific molecular information from the sample, intraoperative IHC is needed.

Lunaphore’s microfluidic technology FFeX is able to dramatically reduce FS IHC turnaround times to an average of 11-15 minutes for FS IHC, compared to the 3 hours of standard automated stainers. With the arrival of LabSatTM, FFeX will open the possibility of performing IHC tests during biopsy procedures while patients are still on the operating table. For research laboratories, an open Research Use Only device, LabSatTM Research, is available.

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