Immunofluorescence (IF) is a staining technique especially used in the research laboratories, which consists in detecting antigens with antibodies fluorescently tagged. The staining can be visualized under a fluorescent microscope.
Fluorescence staining gives a lot of opportunities for research since using different microscope filters, several markers can be differentiated on the same tissue section. In addition, its signal intensity can be quantified giving information on the amount of antibodies present on the sample surface. However, the fluorescence intensity fades down over time and the samples can therefore hardly be re-examined if not scanned and stored as an image, restricting its use so far in the medical settings.
Main focus: Research laboratories – University, Pharma/biotech research labs.
IF tests using Lunaphore
Sample types: Frozen sections/FFPE
Staining time: 5 – 15min